High-throughput inducible expression of transgenes at the Hprt gene in mouse embryonic stem cells.

tion of the cystic fibrosis gene: chromosome walking and jumping. Science 245:1059-1065. 5.Tsui, L.C. 1992. Mutations and sequence variations detected in the cystic fibrosis transmembrane conductance regulator (CFTR) gene: a report from the Cystic Fibrosis Genetic Analysis Consortium. Hum. Mutat. 1:197-203. 6.Ballabio, A., R.A. Gibbs, and C.T. Caskey. 1990. PCR test for cystic fibrosis deletion. Nature 343:220. 7.Liu, Q., E.C. Thorland, J.A. Heit, and S.S. Sommer. 1997. Overlapping PCR for bidirectional PCR amplification of specific alleles: a rapid one-tube method for simultaneously differentiating homozygotes and heterozygotes. Genome Res. 7:389-398. 8.Sarkar, G. and S.S. Sommer. 1990. The “megaprimer” method of site-directed mutagenesis. BioTechniques 8:404-407. 9.Sarkar, G. and S.S. Sommer. 1992. Doublestranded DNA segments can efficiently prime the amplification of human genomic DNA. Nucleic Acids Res. 20:4937-4938. 10.Kerem, B.S., J. Zielenski, D. Markiewicz, D. Bozon, E. Gazit, J. Yahav, D. Kennedy, J.R. Riordan, et al. 1990. Identification of mutations in regions corresponding to the two putative nucleotide (ATP)-binding folds of the cystic fibrosis gene. Proc. Natl. Acad. Sci. USA 87:8447-8451.